This week Naomi Ngendo (sanitation program manager) completed sampling from six MSABI ecological sanitation (eco-san) latrines in Namawala. This is an initial study to determine the condition and safety of the compost. In the laboratory we developed a protocol for testing the sludge and compost samples for thermotolerant faecal coliforms and percentage moisture.
|Faecal sludge sample collected from one of Eco-sans. The mainly vegetarian diet of the villagers gives the faeces a distinct green colour.|
|10 g of sample was blended with sterile water and then serially diluted for microbiological testing.|
|Fatuma Matwewe (laboratory technician) processing samples using membrane filtration method for thermotolerant faecal coliforms.|
|Filters are incubated at 44 oC for 18 h on membrane lauryl sulphate broth. Thermotolerant faecal coliforms are the distinct yellow colonies.|
|To determine the percentage moisture 50 g of sample was dried in an oven at 110 oC for 24 h.|
|Fatuma Matwewe (laboratory technician) mixing the samples to ensure consistent drying prior to weighing.|
|Dried samples reveal a large amount of organic matter which indicates that the household was using this latrine for green solid waste as well.|